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PSB

Carl-Ivar Brändén building (CIBB)
71, Avenue des Martyrs
CS 90181
F-38042 Grenoble
Intranet

Cell Free expression

Purpose of the platform (operated by IBS)


The Cell Free expression platform of IBS is devoted to large scale production (milligrams quantities) of soluble proteins, membrane proteins and RNAs for structural studies (X-ray, NMR...).

We propose the following services:

- Small scale protein expression screening for optimisation of protein constructs. Optimisation of reaction conditions for large scale expression.
- Small scale RNA expression screening. Optimisation of reaction conditions for large scale expression.
- Training courses in RNAse Free wet-lab are proposed to new Cell Free platform users.


Responsible

Jérôme Boisbouvier


Staff in charge of the platform

Platform engineer « Lionel Imbert »
Tel: +33 (0)4 38 78 26 06


In Practice

- Location: IBS - Room 5242

- Access mode: The platform is accessible to local PSB users as well as national and international (Instruct) users. Completed Request form resquest form need to be sent to platform engineer. Upon agreement of the project request by a steering committee, the platform engineer will define, in collaboration with external users, the most adapted strategy and schedule for protein/RNA cell free expression.

- Cost:
Academics: Participation to the RNAse Free wet-lab consumables.
Industrials: Please contact us for cost information.