Focus areas include host-pathogen interaction, membrane protein, extreme environments and fundamental cellular processes such as transcription. Although basic research related to human health is a key program, developing new methodologies and techniques for the future of integrated structural biology is also a major issue for the PSB scientists.
There are currently twelve technical platforms at the PSB ranging from protein expression to biophysical methods based on large facilities.
Standard protein expression (Robiomol)
Expression of difficult proteins (ESPRIT)
Multiprotein complex production using advanced baculovirus technologies (EEF)
Isotopic labelling for neutron or NMR studies
Molecular interaction studies by SPR
Sample quality control (MS, sequencing, NMR, EM)
in cristallo spectroscopy studies (Cryobench)
Biophysics Platform (MALLS, ITC, DLS, etc..)
Robotic nanovolume crystallisation (screening and optimisation)
Synchrotron X-ray crystallography
Small angle X-ray and neutron scattering SAXS/SANS
Negative-stain and cryo- electron microscopy
High Field NMR