Job and training offers:
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Post-doctoral position in virology in Grenoble, France
A post-doctoral position starting on 1 January, 2011 is available for the study of the viral immunosuppressive protein BARF1. It is funded by FINOVI for one year.
We want to establish the role of BARF1 protein in Epstein-Barr virus infection. The structure of this protein has been solved in our group (Tarbouriech et al, 2006) and we could establish the very tight interaction with the human cytokine CSF1 and the structure of the complex (unpublished work). At first, production of BARF1 by different EBV infected cell lines will be studied. Secondly BARF1 will be studied ex vivo (quantification of mRNA with real time PCR, protein detection by enzyme immuno assay, western blot and/or mass spectrometry) in human body fluids from patients with EBV associated diseases and in healthy EBV infected individuals.
The position requires experience in virology at the cellular level. Required techniques are cell culture (especially lymphoblastoïd cell lines), ELISA, immunofluorescence and some experience in molecular biology (real time PCR) and recombinant protein production (in E. coli and in insect cells using baculovirus).
Our group works on the structural biology and physiopathology of Epstein-Barr virus. The group is part of the Institute of Host-Virus Interactions UVHCI, a joint laboratory between the EMBL Grenoble outstation, CNRS and the Grenoble University involving as well the Grenoble University Hospital. Working languages are French and English. Grenoble is a mid-size town with an international scientific atmosphere located in the French Alps.
Deadline: 1 October 2010.
Contacts: Prof. Patrice Morand and Prof. Wim Burmeister
Further information: http://www.uvhci.fr
DATE OF AVAILABILITY : starts as soon as possible, for 2 years (ANR contract).
TITLE : Purification and crystallization of ABC transporters
POSITION OPENED: The position is opened at the Institute for Structural Biology (IBS) in Grenoble in the team of Jean-Michel Jault and will be carried out in the frame of a collaborative project between chemists and biologists, both in Lyon (IBCP and UCB) and in Grenoble (IBS and UJF) and funded by an ANR grant.
JOB DESCRIPTION: MultiDrug Resistance (MDR) is an increasingly challenging problem in the biomedical field and is widespread from tumour cells to microbes. In bacteria, resistance to multiple antibiotics is often associated with the presence of MDR pumps and some of them belong to the largest superfamily of membrane proteins, the ABC (“ATPBinding Cassette”) transporters, which includes some human members whose dysfunction is involved in severe pathologies (cancer, cystic fibrosis, adrenoleukodystrophy…). This project will focus on two bacterial ABC transporters that we routinely purify in high yield and in a functional state, with the goal to crystallize them and solve their 3-D structure, using both classical detergents and newly synthesized ones.
JOB REQUIREMENTS: the applicant should hold a PhD in Biochemistry and have a strong background in protein biochemistry (purification and biochemical/biophysical characterisation) and in protein cristallography. Additional knowledge in membrane protein will be appreciated.
SCIENTIFIC ENVIRONMENT: the Institute for Structural Biology is located in the “Polygone Scientifique” in Grenoble, nearby the EMBL outstation, the ESRF and the ILL, which is one of the most renowned scientific area in France for biological studies. At the IBS, the post-doc will benefit from a technical environment devoted to purification, characterization and crystallization of membrane proteins (MP3, PAOL and high throughput membrane protein crystallization platforms) as well as the scientific expertise associated with. For more information see: http://www.psb-grenoble.eu/ or http://www.ibs.fr/?lang=en
TO APPLY: Please send a CV and the names of two referees to Jean-Michel Jault : jean-michel.jault@ibs.fr
