The EEF is an EMBL installation in the CIBB building which uses MultiBac-EMBL, our state-of-the-art baculovirus expression system for multiprotein complex production developed by the Berger Group.
Introduction into baculovirus/insect cell expression
Introduction into our MultiBac-EMBL system for multiprotein complex production
Multigene expression cassette generation
Composite baculovirus generation
Virus generation and amplification
Viral performance and protein production monitoring
Smale-, medium-, large scale protein (complex) expression
Analytical scale complex identification (if His-tag is used)
| Our MultiBac platform is particularly successfull for high-level production of kinase complexes, virus-like proteins (VLPs), nuclear factors and many other eukaryotic complexes. |
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Imre Berger
Phone: 33 (0)4 76 20 76 61
EMBL Grenoble, BP 181, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
Location: CIBB S07
How to make a request ?: contact Imre Berger
Access Mode: Training and Collaboration
Cost for the PSB users: For training: Reimbursement for media and materials. Collaborations: no costs
Additional Information: [e.g. time scales, involvement expected if collaboration] We are involved in several European programs to train users in baculovirus/insect cell expression by using our MultiBac-EMBL method. We typically do not train more than one user group simultaneously. Therefore, early registration is recommended.
Reference and acknowledgement for publications: "We thank Fred Garzoni from the Berger Group at EMBL Grenoble for assistance and helpful discussions.”
