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PSB

Carl-Ivar Brändén building (CIBB)
71, Avenue des Martyrs
CS 90181
F-38042 Grenoble
Intranet

RoBioMol

Purpose of the platform (operated by IBS)

Molecular Biology and Protein Expression Automated Platform

RoBioMol does the following steps using robots and dedicated software:

- Design two oligos for PCR amplification of selected gene
- Clone gene into a choice of expression vectors
- Sequence two positive clones
- Fractionate expression strain
- Small‐scale affinity purification (insoluble and soluble fractions)
- Analyses purified protein fractions using SDS‐PAGE and Western blot
- Provides a detailed report (with sequence validation of the gene)
- Delivers the expression system (plasmid)


Web page of the platform

webpage


Responsible of the platform

Thierry Vernet


Staff in charge of the platform

Anne-Marie Villard


In practice

Access mode: Service and collaborations

How to make a request: e-mail to robiomol@ibs.fr to
Collaborations:
Upon selection by a scientific committee. For selection criteria and application procedure please contact vernet@ibs.fr
Acknowledgements in publication:
“This work used the platforms of the Grenoble Instruct centre (ISBG ; UMS3518 CNRS-CEA-UGA-EMBL) with support from FRISBI (ANR-10-INBS-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB).
We thank Anne Marie Villard and Dr. Marjolaine Noirclerc-Savoye, for the “gene cloning, site-directed mutagenesis, protein purification or Thermal Shift Assay experiments ; and Céline Charavay and Stéphane Segard (GIPSE, CEA, Grenoble) for the RoBioLIMS database development”.