RoBioMol does the following steps using robots and dedicated software:
Design two oligos for PCR amplification of selected gene
Clone gene into a choice of expression vectors
Sequence two positive clones
Fractionate expression strain
Small‐scale affinity purification (insoluble and soluble fractions)
Analyses purified protein fractions using SDS‐PAGE and Western blot
Provides a detailed report (with sequence validation of the gene)
Delivers the expression system (plasmid)
Benoît Gallet, Tel.: 04 38 78 92 12
Marjolaine Noirclerc,
Tel.: 04 38 78 56 34
Location: IBS-LIM room 7206A
Access mode: Service and collaborations
Service cost for PSB users:
Gene cloning and protein expression/purification test (minimum 24 cloning in the same vector and expression/purification test)
Gene cloning only (minimum 24 cloning in the same vector)
Site-directed mutagenesis (24 mutants minimum)
How to make a request: e-mail to robiomol@ibs.fr to obtain the access password to the robiomol.ibs.fr server
Collaborations:
Upon selection by a scientific committee. For selection criteria and application procedure please contact vernet@ibs.fr
References:
If you publish using reagents produced by the RoBioMol platform please refer to the "RoBioMol platform at the IBS (CEA/CNRS/UJF), Grenoble" both in the methods and the acknowledgements section.
