The Cellular Imaging platform grants the access to a confocal microscope (spinning disk S-M4D), a video-microscope (V-M4D), and a flow-cytometer (VYB). The instruments are optimized to study living cells.
1 - The confocal microscope (S-M4D; Olympus and Andor) is configurated with a Nipkow wheel (Yogokawa CSU-X1) and 6 solid state lasers for confocal imaging in real time on living cells (very low phototoxicity). Images acquisition are done simultaneously with 2 EMCCD cameras (Andor iXon ultra). A photoconversion/photoactivation module is available.
2 - The video-microscope (V-M4D, Olympus and Perkin Elmer) is, as for the confocal composed of an inverted Olympus IX81 fully motorized microscope equipped with a piezo stage, and an incubation chamber set at 37°C for live imaging. Acquisition is achieved with a sCMOS camera (Hamamtsu Orca Flash4; Volocity)
3 - The flow-cytometer (VYB, Miltenyi biotech) is equipped with three lasers and 8 fluorescence channels. It is optimized for fluorescent proteins, and covers all the emission wavelengths used with microscopy.It is automatable (multiwells plate, immuno-staining, dilutions), and compatible with magnetic columns Miltenyi (isolation of rare events).
4 - Cytometry and microscopy analysis software are available for users in a dedicated room. Three workstations are installed with Volocity and Imaris for microscopy data visualisation and analysis; or MacsQuant and CellQuest for flow cytometry.
5 - Super-resolution PALM/STORM microscope. This is a noncommercial in-house built setup based on a microscope (Olympus IX81), 2 EMCCD cameras (Photometrics) and 5 lasers (405 nm, 488 nm, 532 nm, 561 nm, 643 nm) controlled by an acousto-optic filter (AOTF). The EMCCD cameras are controlled through the Metamorph software, the lasers are controlled through LabVIEW. More details on the IBS web site.
– Location: room 547 in the IBS building
– Access mode: Authorized users can access freely the equipment of the platform (microscope and post-treatment dedicated computer), after they have validated the internal training session, and accepted the conditions of use.
For more complex projects or if a specific expertise is requested (collaboration), we can provide a technical assistance for some or all of the experiments planned (observation and post-treatment).
– Cost: Users are charged for the operation costs calculated per hour . Contact the staff in charge of the platform for further information.
