Biophysical Platform

1.Purpose of the Platform

The biophysics platform

The ISBG biophysics platform provides a set of instruments for the in vitro characterization of the intrinsic properties of biological macromolecules in solution, biomolecular interactions and their assemblies:

• - Size, homogeneity and aggregation of macromolecules
• - Oligomeric states and macromolecular assemblies
• - Stoichiometry of macromolecular assemblies
• - Characterization of molecular interactions

The platform is managed by scientists, engineers and technicians with technical and methodological know-how and expertise to assist any scientist in addressing various biological questions and projects.
The biophysics platform is composed of 13 instruments located in different buildings on campus: at IBS, CIBB and EMBL

Isothermal Titration Calorimetry (iTC200 and VP iTC)
Microscale Thermophoresis (MST) Monolith NT115 green/red
Surface Plasmon resonance (SPR: Biacore T200)
Biolayer interferometry (BLI: Octet Red 384)
Fluorescence spectroscopy (steady state or stopped flow)
Analytical ultracentrifugation (AUC)
Dynamic light scattering (DLS) cuvette
Static multi-angle light scattering ( MALS)
Nanoparticle Tracking Analysis (NTA)
Mass Photometry (MP one)

2.Webpage of the Platform

Please visit the ISBG Biophysics web page

3.Responsible

Contact:
caroline.mas@ibs.fr (MALLS, DLS, Fluo, ITC, Mass Photometry, Thermophoresis)
jean-baptiste.reiser@ibs.fr (SPR, BLI)
christine.chatellard@ibs.fr (NTA)
christine.ebel@ibs.fr (AUC, MALLS)
aline.leroy@ibs.fr (AUC, MALLS)