We are glad to announce 2 major pieces of equipment have been integrated at the M4D platform.
Since February this year, the cell imaging platform benefited from the EMBL support to offer users access to cell-sorting. The TYTO (https://www.miltenyibiotec.com/FR-en/products/macsquant-tyto.html) is a versatile cell sorter offering the possibility to open new directions within the research carried out at the PSB. Its original way of sorting involves the use of cartridges containing all the microfluidics required for autonomous and perfectly sterile sorting. In addition, it has the particularity of being able to sort at low pressure, limiting dilution of the cells, which is important to keep cells alive, particularly for fragile populations. These aspects taken together allow for the sorting of almost any cell types, from bacteria to primary human cells, with high efficacy and user friendliness.
Sorting can be combined with a Dispencell (Seed Bioscience – https://seedbiosciences.com/), for single cell dispensing in plates offering the possibility to isolate clonal population and paving the way for single-cell applications. The Dispencell module can be used independently, and involves the use of easy-to-use kits and works at very low pressure.
In parallel, thanks to the financial support of the I2SR group associated with M4D industrial benefits, we upgraded the confocal microscope (S-M4D) to integrate a pulsed 2 kW photo-ablation laser (GATACA™ – https://www.gataca-systems.com/products/gataca-products/ilas-pulse/). This device can create focused damages to any part of a cell in real time, in a manual or automated way. Photo-ablation can be used to create damages to the molecules located at the focus point of the beam, allowing accurate generation of damages. In figure 2, the laser is focused at the membrane level of HEK cells to generate punctual damages in real time, triggering mCherry fused protein relocalization to the damage site. This laser makes also the confocal system compatible with micropatterning applications.
Please feel free to contact the M4D platform staff (using our direct emails or ibs-plateforme-m4d.contact@ibs.fr to discuss projects and get trained for using these setups.
M. Pelosse(EMBL), O. Glushonkov (ISBG), R.-L. Revel-Goyet (IBS), J.-P. Kleman (IBS)
The TYTO in EMBL 158 besides a biosafety cabinet containing the Dispencell.
Photo-damage of HEK membranes and relocalization of the mCherry fused protein to the damaged point in real-time imaging – bar 5µm (N. Miguet IBS/EBEV 2024)
