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EMBO Practical Course – Time-resolved macromolecular serial crystallography

Event date : 8-12 July 2024

Course overview

The advent of serial crystallography at free electron laser sources and the following adaptation of these methods at 3rd and 4th generation synchrotrons have opened new possibilities to perform room temperature time resolved studies in macromolecular crystallography.

This course aims to train the next generation of researchers in

i) sample preparation: Participants will be trained and taught in the best practice to obtain homogenous microcrystals and in characterisation prior to loading using different sample delivery methods,

ii) crystal delivery methods and data collection at a 4th generation synchrotron serial crystallography beamline: The new ID29 and similar endstations at other sources, presents experimental setup and data collection protocols that are unique in the synchrotron facilities. The training will include data collections with different sample delivery methods as available to the user community,

iii) data reduction: Diffraction data are recorded from still microcrystals with large bandwidth high flux X-ray beams which requires the use of specifically developed and adapted software.

iv) analysis of the electron density map differences: To correctly interpret and model the electron density differences map to highlight structural changes induced by pump-probe and ligand mixing experiments.

For further information about the course and the programme: https://www.embl.org/about/info/course-and-conference-office/events/ser24-01/