Assoc. Prof. Magnus Andersson, Department of Chemistry, Umeå university, Sweden. https://www.umu.se/en/research/groups/magnus-p-andersson-lab/
Talk abstract
P-type ATPase transport is carried out in a complex lipid environment and is regulated by both external and internal factors. We have developed a methodology to track P-type ATPase domain rearrangements in real time triggered by laser-induced release of ATP and subsequent monitoring by synchrotron X-ray pulses. We used this time-resolved X-ray solution scattering (TR-XSS) technique to capture the dynamics of eukaryotic and prokaryotic calcium transport (SERCA and LMCA1). We then explored regulatory dynamics induced by the surrounding lipid/detergent environment. We developed structural refinement approached based on MD simulations and ensemble learning algorithms. The results show both subtle and drastic effects on the protein dynamics and kinetics induced by lipid/detergent regulatory processes.
