RoBioMol does the following steps using robots and dedicated software:
– Design two oligos for PCR amplification of selected gene
– Clone gene into a choice of expression vectors
– Sequence two positive clones
– Fractionate expression strain
– Small†scale affinity purification (insoluble and soluble fractions)
– Analyses purified protein fractions using SDS†PAGE and Western blot
– Provides a detailed report (with sequence validation of the gene)
– Delivers the expression system (plasmid)
Access mode: Service and collaborations
How to make a request: e-mail to robiomol@ibs.fr to
Collaborations:
Upon selection by a scientific committee. For selection criteria and application procedure please contact vernet@ibs.fr
Acknowledgements in publication:
“This work used the platforms of the Grenoble Instruct centre (ISBG ; UMS3518 CNRS-CEA-UGA-EMBL) with support from FRISBI (ANR-10-INBS-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB).
We thank Anne Marie Villard and Dr. Marjolaine Noirclerc-Savoye, for the “gene cloning, site-directed mutagenesis, protein purification or Thermal Shift Assay experiments ; and Céline Charavay and Stéphane Segard (GIPSE, CEA, Grenoble) for the RoBioLIMS database development†.
