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PSB

Carl-Ivar Bränden building (CIBB)
71, Avenue des Martyrs
CS 90181
F-38042 Grenoble
Intranet

RoBioMol

Purpose of the platform (operated by IBS)

Molecular Biology and Protein Expression Automated Platform

RoBioMol does the following steps using robots and dedicated software:

 Design two oligos for PCR amplification of selected gene
 Clone gene into a choice of expression vectors
 Sequence two positive clones
 Fractionate expression strain
 Small†scale affinity purification (insoluble and soluble fractions)
 Analyses purified protein fractions using SDS†PAGE and Western blot
 Provides a detailed report (with sequence validation of the gene)
 Delivers the expression system (plasmid)


Web page of the platform

webpage


Responsible of the platform

Thierry Vernet


Staff in charge of the platform

Anne-Marie Villard


In practice

Access mode: Service and collaborations

How to make a request: e-mail to robiomol@ibs.fr to
Collaborations:
Upon selection by a scientific committee. For selection criteria and application procedure please contact vernet@ibs.fr
Acknowledgements in publication:
“This work used the platforms of the Grenoble Instruct centre (ISBG ; UMS3518 CNRS-CEA-UGA-EMBL) with support from FRISBI (ANR-10-INBS-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB).
We thank Anne Marie Villard and Dr. Marjolaine Noirclerc-Savoye, for the “gene cloning, site-directed mutagenesis, protein purification or Thermal Shift Assay experiments ; and Céline Charavay and Stéphane Segard (GIPSE, CEA, Grenoble) for the RoBioLIMS database development†.