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PSB

Carl-Ivar Brändén building (CIBB)
71, Avenue des Martyrs
CS 90181
F-38042 Grenoble
Intranet

High Throughput Membrane Protein Crystallization Platform

Purpose of the platform (operated by IBS)


The High Throughput Membrane Protein Crystallization (HTMPC) platform is fully equipped to accomplish successful crystallization of your membrane protein (in meso, bicelle, vapor diffusion). Robotic systems nanovolume dispenser (NT8 from Formulatrix) allows to screen 96 precipitant conditions with small amount of sample (5-10 µl per plate) in less than 10 minutes. Our platform works with commercially available kits specialized in membrane protein crystallization. An automated imaging system, RockImager 1500 (Formulatrix), visualizes crystallization drops with visible, polarized and UV lights, which allows a better identification of protein crystals. The smallest crystals which can be confidently detected are about 10 micrometers.

The diffusion rate and mobile fraction of the membrane protein can be determined by FRAP (Formulatrix), that allows to identify the optimal conditions for LCP crystal growth. In addition, FTIR (Brucker) and UV-Vis specrophotometer (Schimadzu) are available at the platform for precise determination of detergent and membrane protein content correspondingly.


Web page of the platform

https://www-crims-htmpc.ibs.fr/
login: crimsUser pwd: 6horowitz


Responsible

Valentin Gordeliy


Staff in charge of the platform

Florine Dupeux
Delphine Blot


In practice

contact: ibs-plateforme-htmpc.contact@ibs.fr
location: IBS, room 313; office 357
acces mode: The platform is accessible to local PSB users as well as national and international users.
Register and send crystallization requests from our website.
cost: https://www-crims-htmpc.ibs.fr/index.php?option=com_content&view=article&id=49&Itemid=395