Sample homogeneity and oligomerisation state of the protein sample
3-D reconstruction of macromolecular assemblies
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Negative staining: The protein or complex has to be bigger than 100 kDa. We need 20 microl at 0.2 mg/ml (+buffer). Try to avoid detergents.
Cryo-EM: only collaboration. The protein or complex has to be bigger than 500 kDa.