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PSB

Carl-Ivar Brändén building
6, rue Jules Horowitz, BP181
F-38042 Grenoble
Intranet

Electron Microscopy

Purpose of the platform (operated by IBS)

- Sample homogeneity and oligomerisation state of the protein sample
- Cell slicing
- 3-D reconstruction of macromolecular assemblies




Web page of the platform


Responsible

Guy Schoehn
or: ibs-plateforme-em.contact at ibs.fr
tel: 04 38 78 45 68


Staff in charge of the platform

- Guy Schoehn ph: 04 38 78 45 68
- Daphna Fenel ph: 04 38 78 92 62
- Maria Bacia ph: 04 38 78 96 12
- Benoit Gallet ph: 04 38 78 92 62


In practice

Location: IBS
For access send an e-mail to ibs-plateforme-em.contact at ibs.frr
Access mode: To be defined : service or collaboration
Cost for PSB-IVMS users: pricing is under progress (see IBS web page)
Negative staining: The protein or complex has to be bigger than 100 kDa. We need 20 microl at 0.2 mg/ml (+buffer). Try to avoid detergents.
Cryo-EM: only collaboration. The protein or complex has to be bigger than 500 kDa.

Availability of the microscopes

- EMBL-JEOL1200
- IBS-CM12
- IBS-CM200
- IBS-POLARA